欧美日本免费一区二_久久99国产亚洲精品观看_精品少妇一区二区三区在线播放_国产成人高清在线_狠狠操首页_好好操欧美_秋霞黄色毛片_久久精品国内热,亚洲精品国精品久久99热一,亚洲日韩精品无码AV,国产av一区二区精品

產(chǎn)品中心您現(xiàn)在的位置:首頁(yè) > 產(chǎn)品展示 > > 特色試劑盒 > cAMP complete ELISA kit

cAMP complete ELISA kit

更新時(shí)間:2022-01-28

簡(jiǎn)要描述:

cAMP complete ELISA kit
Most sensitive (0.006 pmol/ml and 0.027 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.

型號(hào):廠商性質(zhì):代理商瀏覽量:972
  • Versatile and convenient kit used to assay both intracellular and extracellular cAMP

  • Highy sensitive measurement of cAMP with an optional acetylation protocol increasing sensitivity >10-fold (0.006 pmol/ml and 0.027 pmol/ml) for intra- and extracellular cAMP, respectively

  • Simple, efficient and well-established sample handling protocols

  • Widely cited in peer-reviewed publications

  • Higher throughput format with results in 3 hours for up to 39 samples in duplicate

  • Reliable and consistent lot-to-lot performance

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
Complete cAMP ELISA kit Standard curve - Non-Acetylated in AB2
Typical standard curve used to calculate cAMP concentrations in Assay Buffer 2 for Non-acetylated assay format.
Please mouse over
Complete cAMP ELISA kit Standard curve - Acetylated in AB2Complete cAMP ELISA kit Standard curve - Acetylated HClComplete cAMP ELISA kit Standard curve - Non-Acetylated in AB2Complete cAMP ELISA kit Standard curve - Non-acetylated in HCl


Product Details

Alternative Name:cyclic AMP

Sensitivity:Non-acetylated: 0.49 pmol/ml (assay buffer) / 1.18 pmol/ml (HCl) (range (non-acetylated) 0.78 - 200 pmol/ml)
Acetlylated: 0.027 pmol/ml (assay buffer) / 0.006 pmol/ml (HCl) (range (acetylated) 0.078 - 20 pmol/ml)

Assay Time:3 hours

Applications:ELISA, Colorimetric detection

Application Notes:For the quantitative determination of cAMP in cell lysates, culture supernatants, serum, saliva, and tissue from any species.

Wavelength:405 nm

Species reactivity:Species independent

Crossreactivity:cyclic AMP (100%)
AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)

Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.

Shipping:Shipped on Blue Ice

Contents:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Assay buffer 2, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Triethylamine, Acetic anhydride

Scientific Background:Cyclic AMP (cAMP) is one of the most important “second messengers" involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Regulatory Status:RUO - Research Use Only

Product Literature References

Inhibition of relaxin autocrine signaling confers therapeutic vulnerability in ovarian cancer: H.E. Burston, et al.; J. Clin. Invest. 131, e142677 (2021), Abstract;
Coupling of melanocyte signaling and mechanics by caveolae is required for human skin pigmentation: L. Domingues, et al.; Nat. Commun. 11, 2988 (2020), Application(s): cAMP quantification in human melanocytes, Abstract; Full Text
Increased salt intake does not worsen the progression of renal cystic disease in high water-loaded PCK rats: S. Nagao, et al.; PLoS One 14, e0207461 (2019), Application(s): ELISA using rat kidney, Abstract; Full Text
Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4: I. Russo, et al.; J. Neuroinflammation 15, 297 (2018), Abstract; Full Text
Prostanoid EP2 Receptors Are Up-Regulated in Human Pulmonary Arterial Hypertension: A Key Anti-Proliferative Target for Treprostinil in Smooth Muscle Cells: J.A. Patel, et al.; Int. J. Mol. Sci. 18, 2372 (2018), Abstract; Full Text
Biventricular differences in β-adrenergic receptor signaling following burn injury: A.N. Guillory, et al.; PLoS One 12, e0189527 (2017), Abstract; Full Text
Donor variability may mask dimethyl fumarate's effects on nuclear factor E2-related factor 2 in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; BMC Res. Notes 10, 553 (2017), Abstract; Full Text
First mutation in the FSHR cytoplasmic tail identified in a non-pregnant woman with spontaneous ovarian hyperstimulation syndrome: J. Hugon-Rodin, et al.; BMC Med. Genet. 18, 44 (2017), Application(s): ELISA using COS-7 cell lysates, Abstract; Full Text
Gαs regulates Glucagon-Like Peptide 1 Receptor-mediated cyclic AMP generation at Rab5 endosomal compartment: S.B. Girada, et al.; Mol. Metab. 6, 1173 (2017), Application(s): Lysates of BRIN-BD11 pancreatic beta cells, Abstract; Full Text
Narciclasine attenuates diet-induced obesity by promoting oxidative metabolism in skeletal muscle: S.G. Julien, et al.; PLoS Biol. 15, e1002597 (2017), Abstract; Full Text
Semi-microbiological synthesis of an active lysinoalanine-bridged analog of glucagon-like-peptide-1: A. Kuipers, et al.; Peptides 91, 33 (2017), Abstract;
Supplement of cilostamide in growth medium improves oocyte maturation and developmental competence of embryos derived from small antral follicles in pigs: H. Lee, et al.; Theriogenology 91, 1 (2017), Abstract;
Astemizole-Histamine induces Beclin-1-independent autophagy by targeting p53-dependent crosstalk between autophagy and apoptosis: R. Jakhar, et al.; Cancer Lett. 372, 89 (2016), Application(s): cAMP assay using cell-conditioned media, Abstract;
Cilostamide and forskolin treatment during pre-IVM improves preimplantation development of cloned embryos by influencing meiotic progression and gap junction communication in pigs: B. Park, et al.; Theriogenology 86, 757 (2016), Application(s): Measurement of cAMP levels in oocytes, Abstract;
Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun. 475, 19 (2016), Application(s): PBMCs, Abstract;
Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase: L. Ramos-Espiritu, et al.; Nat. Chem. Biol. 12, 838 (2016), Application(s): Measurement of intracellular cAMP; Assessing reproducibility and concentration dependency, Abstract;
Modulation of breast cancer cell viability by a cannabinoid receptor 2 agonist, JWH-015, is calcium dependent: K.E. Hanlon, et al.; Breast Cancer (Dove Med. Press.) 8, 59 (2016), Application(s): Used to measure forskolin-induced cAMP formation in vitro in response to JWH-015, Abstract; Full Text


如需購(gòu)買或咨詢?cè)摦a(chǎn)品技術(shù)問題,請(qǐng)聯(lián)系ENZO一級(jí)代理商-欣博盛生物

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
男女性爱视频一区二区三区| 九色在线蜜臀| 黄色片AV| 超级福利精品| 99精品一区二区三区不卡 | 欧美一区二区三区精品.人妻| 午夜福利看片一区二区三区四区| 亚洲国产午夜精品大秀视频| 久久久美女裸身露逼免费视频无遮挡| 亚日韩AV一区| 国产高清无密码一区二区三区| 国产三级在线观看视频| 操逼操逼操逼视频操逼| 小泽玛利亚视频一区二区| 99热这里只有成人精品国产| 欧美国产综合一区| 久久艹3| 人妻13p| 超碰91在线| 国产精品福利高清hd| 日韩午夜啪啪视频| 抽插 一区二区| 中文字幕一区二区三区人妻久久| 色婷婷在线一区二区三区| 国产家庭乱伦专区视频| 使劲干福利导航| 97se亚洲国产综合自在线| 亚洲精品乱码久久久久久97 | 高清欧美日韩一区二区三区在线观看| 丰满少妇内射一区| 日本极品综合网| 国产999在线| 国产精品永久免费| 欧美一级特黄特色大片免费| 香蕉超碰| 亚洲男女av| 欧美日韩亚洲另类综合| 真正欧美日韩性爱| 久久精品人妻中文系列亚洲人妻| 国产免费一区二区三区香蕉精| 91日韩|